Applications of interactomics using Genomics and proteomics technologies
ABOUT THE COURSE:
Due to rapidly evolving technological platforms in biology, there is a need to keep pace with latest developments in field to explore their versatile applications. Interactions resulting from protein-protein, protein-peptide, protein-RNA, protein-DNA or protein- small molecule have immense application in life-sciences and translational biology. Through this course, we aim to provide an interface between distinguished scientists involved in advanced interactomics research, industrial partners, faculties and students. This course would feature an intensive lecture series followed by some demonstrations designed to provide the much needed training required to explore the endless possibilities in interactomics research using genomics and proteomics approach, that can be useful for a researcher at any stage.This course of applications of interactomics using genomics and proteomics technologies focuses on two powerful platforms: Protein microarrays and Label-free biosensors and Next-Generation Sequencing (NGS) Technology.The students/participants will gain exposure to the above mentioned technologies and its applications from some of the experts in the field, from both- academia and industry perspective.
INTENDED AUDIENCE: Biology or biotechnology background students having interest in latest technologies, (BE/B.Tech) Biotechnology Students, B.Sc. students science background but course is open to all.
CORE/ELECTIVE: Elective
UG/PG: UG/PG
PREREQUISITES: None
INDUSTRY SUPPORT: GE Healthcare, Pall Life Sciences, Thermofisher Scientific, Illumina
1521 students have enrolled already!!
ABOUT THE INSTRUCTOR:
Prof. Sanjeeva Srivastava is an Assistant Professor in Department of Bioscience and Bioengineering at Indian Institute of Technology Bombay, Mumbai. Proteomics describes the study and characterization of complete set of proteins present in a cell, organ or organism at a given time. My laboratory is using high throughput proteomic techniques such as mass spectrometry and protein microarray etc. for biomarker discovery in cancer & tropical diseases of India, to study protein-protein interactions and drug target discovery. Information obtained from research program is also used for in silico studies and computing models to enhance our understanding in systems approach.
Post-doc, Harvard Institute of Proteomics, Harvard Medical School, USA (2007-2009)
Assistant Professor, Department of Biosciences and Bioengineering, IIT Bombay (2009-2014)
Associate Professor, Department of Biosciences and Bioengineering, IIT Bombay (2014-onwards)
COURSE LAYOUT:
Week 1 : Introduction to Interactomics and Protein Arrays ,Basics and Applications of Reverse Phase Protein Arrays-I,Basics and Applications of Reverse Phase Protein Arrays-IIBasics and Applications of Reverse Phase Protein Arrays-III,Weekly Perspective-I Week 2 : NAPPA Technology and Protein Arrays , NAPPA Technology and Protein Arrays-II, Using functional proteomics to identify biomarkers and therapeutic targets , Using functional proteomics to identify biomarkers and therapeutic targets-II, Weekly Perspective-II Week 3 : NAPPA Arrays: Workflow, NAPPA and its applications in study of antibody immune response in disease and in drug screening-I, NAPPA and its applications in study of antibody immune response in disease and in drug screening-II, NAPPA and its applications in study of antibody immune response in disease and in drug screening-III, Weekly Perspective -III Week 4 : Biomarkers: Harnessing the immune system for early detection of disease-I, Biomarkers: Harnessing the immune system for early detection of disease-II, Biomarkers: Harnessing the immune system for early detection of disease-III, Multi-variate data analysis to identify potential leads from proteomic datasets, Multi-variate data analysis to identify potential leads from proteomic datasets Week 5 : The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-I , The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-II, Antibody signatures defined by high-content peptide microarray analysis Week 6 : An overview of label-free technologies-I, An overview of label-free technologies-II, Mass Spectrometry coupled Interactomics-I, Mass Spectrometry coupled Interactomics-II, Biomolecular interactions using Bio-Layer Interferometry (BLI) Week 7 : Surface Plasmon Resonance- Principles and Assays-I, Surface Plasmon Resonance- Principles and Assays-II, SPR Application, Biomolecular interaction analytics using MicroScale Thermophoresis , NGS target enrichment workflow for exomes, targeted panels and beyond Week 8 : Next-Gen Sequencing Technology-I, Next-Gen Sequencing Technology-II, Next-Gen Sequencing Technology-III, Next-Gen Sequencing Technology-IV , Summary of high-throughput technologies to study DNA and protein interactions
SUGGESTED READING MATERIALS:
Proteomics: A Cold Spring Harbor Laboratory Course Manual, A.J. Link and J. LaBaer, Cold Spring Harbor Laboratory Press, 2009.
CERTIFICATION EXAM :
The exam is optional for a fee.
Date and Time of Exams: April 27 2019(Saturday) Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
Registration url: Announcements will be made when the registration form is open for registrations.
The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published.
CERTIFICATION:
Final score will be calculated as : 25% assignment score + 75% final exam score
25% assignment score is calculated as 25% of average of Best 6 out of 8 assignments
E-Certificate will be given to those who register and write the exam and score greater than or equal to 40% final score. Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Bombay.It will be e-verifiable at nptel.ac.in/noc.
