This course
introduces to the basic biology of proteins and the new advanced science called
as proteomics which aims to look into the protein properties from a global
perspective, i.e., not undertaking one protein at a time, but an entire set of
proteins in the milieu. The course will cover in detail the two major aspects
of proteomics i.e., Gel-based proteomics and Mass spectrometry-based
proteomics. The gel-based module will cover different techniques like SDS-PAGE,
2-DE, 2D-DIGE etc. These techniques had a major contribution in transition from
protein chemistry to proteomics. Mass spectrometry, on the other hand, is an
advanced analytical technique for accurate mass measurement. In this module, we
will discuss the basics of mass spectrometry, sample preparations, liquid
chromatography, hybrid mass spectrometers and quantitative proteomics
techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course
will also provide the basic knowledge about sample preparation, mass
spectrometry workflow, different chromatography technologies and quantitative
proteomics.
Intended Audience :
Core course. All UG and PG students can attend this
course. It would be applied to B.Sc., M.Sc. and MS.Pre-requisites :
Any B.Sc. Or M.Sc.The target audiences of this course are required
to have a basic introduction to biology.